Mass spectrometry an analytical chemistry technique identify and quantify molecules in simple and complex mixtures measures the masstocharge ratio and abundance of gasphase ions. Winner of the standing ovation award for best powerpoint templates from presentations magazine. The mclafferty rearrangement is a reaction observed in mass spectrometry during the fragmentation or dissociation of organic molecules. Quantitative malditof mass spectrometry for clinical applications.
A history of mass spectrometry in australia downard 2005. Overview of mass spectrometry for metabolomics thermo. The nature of these components varies based on the type of mass spectrometer, the type of data required, and the physical properties of the sample. Block diagram of a mass spectrometer ion source detector high vacuum sample in computer tandem mass spectrometry or msms mz. O connor powerpoint ppt presentation free to view mass spectrometry market mass spectrometry ms is a systematic technique that ionizes chemical species and classified the ions on their masstocharge ratio. See more ideas about mass spectrometry, chemistry jokes and chemistry humor. Mass spectrometry is revolutionising the scientific understanding of matter allowing for breakthroughs in the fields of astronomy, biology, material science and medicine. Peacock, open source, mac os x application developed by johan kool that can be used to interpret. Mass spectrometry ms is an analytical technique that measures the mass tocharge ratio of charged particles.
That means, you can run compounds together in one run with the ability to control the quadrupole resolution per transition to get the best sensitivity and avoid running two different methods. The operating principle and key applications of mass spectrometry. This resource can be used as revision material or if you are pressed with time teaching this topic. Students may use windows, mac osx or linux environments. A letter to astonearly mass spectrometry in australia. It is used for determining masses of particles, for determining the elemental composition of a. The complete process involves the conversion of the sample into gaseous ions, with or without fragmentation, which are then characterized by their. In the spectrum of propane the peak at 29 is the base peak most abundant 100%. Each primary product ion derived from the molecular ion, in turn, undergoes fragmentation, and so on. Spectroscopy involves an interaction between matter and 962537 ppt. The ions encounter electric andor magnetic fields from mass analyzers, which deflect the paths of individual ions based on their mz. All mass spectrometers that you will come across if you are doing a course for 16 18 year olds work with positive ions.
Msacl 2016 us annual congress in clinical mass spectrometry. A mass spectrum is the plot of relative abundance of ions against their masscharge ratio. In the source region, neutral sample molecules are ionized and then accelerated into the mass analyzer. Overview of mass spectrometry thermo fisher scientific br. Use dedicated tools to generate molecular formula from given mass or compound. Mass spectrometry is used to confirm the identify of unknowns, such as illegal drugs. Jul 25, 2012 the pattern of molecule sizes and amounts is characteristic of a particular material. The ionizer converts a portion of the sample into ions.
Maxquant is one of the most frequently used platforms for massspectrometry ms based proteomics data analysis. For the determination of molecular formula by mass spectrometry, it is essential to identify the molecular ion peak as well as its exact mass. Mar 20, 2016 as level work on the mass spectrometer, covers parts of unit 1 and unit 2 wjec new specification. Discussions will include the optimization of sample preparation for metabolite extraction and protein removal, as well as choosing the most appropriate. Mass spectrometry 1 mass spectrometry 2 gas chromatogram of flavor compounds 3 definition a mass spectrometer bombards a substance under investigation with an electron beam and quantitatively records the result as a spectrum of positive ion fragments. A mass spectrometer or mass spectrograph consists of three essential parts. Richard caprioli, and has continued to garner interest in labs around the world as its applications expand mcdonnell and heeren, 2007b, reyzer and caprioli, 2011.
Biomedical application of maldi mass spectrometry for small. Mass spectrometry introduction general overview mass spectrometry is the generation, separation and characterization of gas phase ions according to their. Figure 1 is a block diagram that shows the basic parts of a mass spectrometer. The total mass of a water molecule is the sum of the mass of two hydrogens ap proximately 1 atomic mass unit per hydrogen and one oxygen approximately. The molecular fragmentation peaks in the mass spectrum can give clues as. Mass spectrometry fingerprints the components, so that they can be matched to existing known spectra. Sometimes the energy needed for exciting the atoms is obtained simply by heating.
A simple description of how a mass spectrometer works. At high energies, the ionising electron beam can fragment the molecule by cleaving particular bonds. The part of the ms called the mass analyzer or deflector so named because the positively charged ions inside will be deflected from their straight paths by a sideways magnetic field is featured in the interactive animation below. Mass spectrometrybased imaging of tissue samples and surfaces is a relatively new technology with a significant function in medicinal development mcdonnell and heeren, 2007b. Ppt mass spectrometry powerpoint presentation free to. Lecture 1 introduction, history, scope and terminology of. Unknowns are often not pure, and must be separated from a mixture. Worlds best powerpoint templates crystalgraphics offers more powerpoint templates than anyone else in the world, with over 4 million to choose from. Apr 01, 2015 the mass spectrometer allows scientists to study and examine the composition of molecules at small scales in a way that was never before possible. The basic aspect of organic mass spectrometry consist of bombarding. New, easytouse analytical detection system extracts meaningful data quickly from acquisition to analysis. The mass spectrometer allows scientists to study and examine the composition of molecules at small scales in a way that was never before possible. The inlet transfers the sample into the vacuum of the mass spectrometer. Advances in structure elucidation of small molecules using mass.
The ionization chamber is a region in which atoms of the unknown material are excited so as to make them lose electrons. A short explanation for the absolute novice what does a mass spectrometer do. Its a peak produced by a molecule that has been ionized. Mass spectrometry introduction, principle of mass spectrometry. Mass spectrometry software is software used for data acquisition, analysis, or representation in. Aqa time of flight mass spectrometer teaching resources. Born in melbourne in 1899, wark was a capable student who was offered a scholarship at the working. Charge deconvolution infers the mass from mass over charge mz measurements in electrospray ionization mass spectra. Even if a few atoms in a sample of chlorine, for example, captured an electron instead of losing one, the negative ions formed wouldnt get all the way through the ordinary mass spectrometer. The molecular fragmentation peaks in the mass spectrum can give clues as to the substructure of the molecule. An introduction to mass spectrometry applications in. The ability of a mass spectrometer to distinguish between ions of different mass. Mass spectrometry ms is an analytical technique that measures the masstocharge ratio of ions.
This work presents a systematic investigation of the molecular level composition and the extent of aqueous photochemical processing in. The nature of these components varies based on the purpose of the mass spectrometer, the type of data required, and the physical properties of the sample. Block diagram of a mass spectrometer ion source mass analyzer detector high vacuum sample in computer 400 600 800 1200 1400 1600 1800mz 0 20 40 60 80 100 relative abundance mass analyzer activation or reaction tandem mass spectrometry or msms. Mass spectrometrybased tissue imaging of small molecules.
The data suggest that wholecell malditof ms has potential as a rapid and. The results are typically presented as a mass spectrum, a plot of intensity as a function of the masstocharge ratio. Ms for the analysis of small molecules and discuss its. The separation chamber of the mass spectrometer is keep under a.
Applications of mass spectrometry in the physical sciences. Timeofflight secondary ion mass spectrometry tofsims has been used for many years to generate molecular images and depth profiles of samples including tissue sections. Protonated molecule, deprotonated molecule, adduct ion, molecular ion low energy process soft ionization produces little fragmentation produces molecular ion apci uses solvents o2 and n2 to produce reagent ions by a corona discharge in atmospheric pressure. The maxquant computational platform for mass spectrometry. Mass spectrometry ms is a technique that measures the molecular masses found in a sample, and reports them in the form of a mass spectrum. Mass spectrometry works by ionizing chemical compounds to generate charged molecules or molecule fragments and measuring their massto. Current and future applications of mass spectrometry to the. Qualitative and quantitative composition of complex samples. S q h l f a p i s e k 200 400 600 800 1200 0 20 40 60 80 100 relative abudance mz. When applied over a wide input mz or broad target mass range, chargedeconvolution algorithms can produce artifacts, such as false masses at onehalf or onethird of the correct mass. We had one running here at the university of rochester for a while. The operating principle and key applications of mass.
Structures of inorganic, organic and biological molecules. It is difficult to identify australias first entry into mass spectrometry, but as reported 16 a communication between the then postgraduate student ian wark and francis aston is among the earliest documented interest in mass spectrometry. Exact mass of an ion or molecule calculated using the mass of the most abundant isotope of each element molar mass. Whether or not you want molecular ions depends on wh.
A free powerpoint ppt presentation displayed as a flash slide show on id. Mass spectrometry free download as powerpoint presentation. This is used for the heavy elements, and makes measurements atom by atom not whole or fragmentary molecules. These will be considered separately and then followed with a series of example applications. Commonly used mass analyzers include timeofflight tof, orbitraps, quadrupoles and ion traps, and each type has specific. There are a variety of different types of mass spectrometer available. Coupled to mass spectrometry ms, it represents a powerful anal. Samples are introduced into the mass spectrometer in liquid or gas form and then vaporized and ionized by the ion. In order to make this measurement from a biologically relevant molecule, there are three essential components to a mass spectrometer. A powerpoint with questions about the time of flight mass spectrometer that links to the information that aqa produced for the new as a2 specification. The charge that these molecules receive allows the mass spectrometer to accelerate the ions throughout the remainder of the system. Indeed, a maximum entropy term in the objective function of maxent, the most commonly used. Lecture 3 mass spectrometry principles of mass spectrometry mass spectrometry separates molecules based on the mass to charge ratio.
Daughter scan parent scan a scan of all parents which produce a particular mass ion. Mass spectrometry body, chemical, energy, gas, parts. Since its first release in 2008, it has grown substantially in functionality and can be used in conjunction with more ms platforms. The mass spectrum of the apple volatiles was published in a tabulated form in 1951 and revealed prominent ions indicative of the presence of esters ethyl and propyl acetate, propionate, n. Mass spectrometer system offers solution for small molecule identification and characterization. It is sometimes found that a molecule containing a ketogroup undergoes. The course covers sample preparation, choice of ionization method and mass spectrometer, as well as data preparation and analysis, structural characterization and biomarker validation. A history of mass spectrometry in australia downard. A mass spectrometer uses charged particle optics or ion optics to direct ionized molecules from an ion source, through a mass analyzer, to an ion detector, determining the molecules mass in the process. Highresolution mass spectrometry and molecular characterization. How a mass spec works most mass spectrometers in use today for biomedical research function by measuring the mass. Samples are loaded into the mass spectrometer in liquid, gas or dried form and then vaporized and ionized by the ion source e. Mikes mass analyzed ion kinetic energy spectrometer, be geometry tof time of flight mass spectrometer qqq a triple quadrupole mass spectrometer scanning modes product scan a scan of the product distribution from a single mass precursor.
A mass spectrometer produces charged particles ions from the chemical substances that are to be analyzed. As level work on the mass spectrometer, covers parts of unit 1 and unit 2 wjec new specification. Cassinihuygens space probe sent to saturn carries a ion and neutral mass spectrometry. Chapter 20 molecular mass spectrometry ms is probably the most widely applicable of all the analytical tools available. Gas chromatography is used to separate the components of the mixture. Included powerpoint and worksheets for students to use as you go along, to help them visualise the different spectra, with plenty of examples to. Spectroscopyspectroscopy basic theory mass spectroscopy deals with the examination of the characteristics fragments ions arising from the breakdown of organic molecules.
Kg, suitable for lcms and gcms data of all kinds of molecules, the analysis of intact mass spectra of proteins, the. Identification of mycobacteria by matrixassisted laser desorption. Stephen master received his undergraduate degree in molecular biology. Mass spectrometry is used in many different fields and is applied to pure samples as well as complex mixtures. A mass spectrometer contains an ion source, a mass analyzer and an ion detector. Mass spectrometer a mass spectrometer converts them to gaseous ions so that they can be moved about and manipulated by external electric and magnetic fields the three essential functions of a mass spectrometer, and the associated components, are. Mass spectrometry serves as the best possible technique for the determination or confirmation of molecular weight of compounds. View and download powerpoint presentations on mass spectrometry ppt. Scientists also use dual sector mass spectrometers.
Mass spectrometer system offers solution for small molecule identification and characterization new, easytouse analytical detection system extracts meaningful data quickly from acquisition to. Mass spectrometers use a given molecule s masscharge ratio mz to provide a means to uniquely identify components present in the sample. Spectroscopy involves an interaction between matter and light electromagnetic radiation light can be thought of as waves of. Find powerpoint presentations and slides using the power of, find free presentations research about mass spectrometry ppt. Another common mass spectrometer is a quadrupole mass spectrometer. Introduction to mass spectrometry ms the xaxis of a mass spectrum represents the masses of ions produced mz. While this course uses powerpoint, the methods taught can be used with almost any presentation software. A mass spectrum is the plot of relative abundance of ions against their mass charge ratio. May 04, 2016 a powerpoint with questions about the time of flight mass spectrometer that links to the information that aqa produced for the new as a2 specification. Thanks for contributing an answer to chemistry stack exchange. The ion source a small sample is ionized, usually to cations by loss of an electron. The mass spectrometer then uses electric and magnetic fields to measure the mass weight of the charged particles.
Mass spectrometry mass spectrometry is a powerful analytical technique used to quantify known materials, to identify unknown compounds within a sample, and to elucidate the structure and chemical properties of different molecules. All mass spectrometers seperate ions based on their mass to charge ratio, and molecules can be ionized and analyzed just like atoms are. Structure elucidation, mass spectrometry, tandem mass. Indeed, a maximum entropy term in the objective function of maxent, the most. This ensures that the ions can travel from the source to the detector without collision. But avoid asking for help, clarification, or responding to other answers. In general, for any two mass spectrometers of the same make and model measurement outcomes will depend on individual instrument tuning, cleanliness, and. Molecular imaging of tissue sections by mass spectrometry ms has become an enabling technology in biological and medical research. The software delivers fullsystem integration and serves as a single point of control for a range of. The mass analyzer is the heart of the mass spectrometer. Mass spectrometer system offers solution for small. This rearrangement may take place by a radical or ionic mechanism.
The separation chamber of the mass spectrometer is keep under a high vacuum and so all. Mass of one mole 6x1023 atoms or molecules of a molecule compound i. Furthermore, from the perspective of providing crosssectional area for a target, the molecule is composed of mostly free. Mass of one mole 6x1023 atoms or molecules of a moleculecompound i. Protonated molecule, deprotonated molecule, adduct ion, molecular ion low energy process soft ionization produces little fragmentation produces molecular ion apci uses solvents o2 and n2 to produce reagent. The instrument must also be connected to a computer system to process and record the data and a vacuum pump to control the pressure within the mass spectrometer see fig. Theyll give your presentations a professional, memorable appearance the kind of sophisticated look that todays audiences expect.